Imigca yeseli ehleliweyo yemfuza
Imigca yeseli ehleliweyo yemfuza ibhekisa kwiiseli eziye zafumana ukuguqulwa kofuzo ngenkqubo eyaziwa ngokuba luhlelo lwemfuza.Ukuhlelwa kwemfuza kubandakanya ukwenza utshintsho kwi-DNA yezinto eziphilayo, rhoqo ngenjongo yokufunda imizila yemfuza ethile okanye ukuqonda iinkqubo ezithile zebhayoloji.
Ukusebenzisa itekhnoloji yokuhlela i-gene, kunokwenzeka ukufezekisa imigca yeeseli zegesi, imigca yeeseli ezikhuphayo, ukuguqulwa kwamanqaku, okanye ukubethelwa kweeseli zeeseli kwiiseli ezithile, ezinokuthi emva koko zisetyenziswe ukufunda umsebenzi wemfuza, umqondiso, iindlela zesifo. , kunye nophuhliso lwamachiza, kunye nokuleyibhela iiseli ezithile.
Kukho izizathu ezininzi zokuba kutheni imigca yeseli ehleliweyo yemfuza ixabisekile kuphando lwezenzululwazi.Okokuqala, babonelela ngesixhobo sokufunda umsebenzi wemfuza ethile.Ngokuguqula imizila yemfuza kwindawo elawulwayo, izazinzulu zinokubona umphumo wokuguqulwa kwemizila yemfuza kwindlela iiseli eziziphatha ngayo okanye ezinye iinkqubo zebhayoloji.Olu lwazi lunokuba negalelo ekuqondeni ngcono izifo zofuzo, kunye nokuchonga iithagethi ezinokubakho zonyango.
Ukongeza, imigca yeseli ehleliweyo ingasetyenziselwa ukuphuhlisa nokuvavanya amachiza amatsha.Ngokuzisa ukuguqulwa kofuzo oluthile, izazinzulu zinokwenza imigca yeeseli ezilinganisa iimeko zesifo, zibenza bakwazi ukuhlola ukusebenza konyango olunokubakho.Oku kuvumela iinkqubo ezichanekileyo nezisebenzayo zophuhliso lwamachiza.
Ngaphezu koko, imigca yeseli ehleliweyo yofuzo inamandla okusetyenziswa kwiyeza lokuhlaziya.Ngokuguqula iijini ezinoxanduva lokuziphatha kweeseli okanye ukwahlulahlula, izazinzulu zinokuphucula amandla onyango eeseli ze-stem okanye zenze iiseli ezilungele ngakumbi ukutshintshwa.
Inzuzo
1. I-Gene-edited multi-locus ngexesha elinye;
2. ukubonelela ngenkonzo yokuma enye ukusuka kuyilo lwe-sgRNA kunye nokudibanisa ukukhangela umgca weseli;
3. Amava atyebileyo kwi-gene synthesis ukunceda i-gene editing vectors ukusuka kuyilo lwesikimu ukuya ekugqityweni kolwakhiwo.
Ishishini eliPhambili
- Gene Knock-out Cell Lines
- Gene Activation Cell Lines
- Point imigca yeseli eziguquguqukayo
- Knock-in Cell Lines
Iimbekiselo
[1] Zhang S, Shen J, Li D, Cheng Y. Amaqhinga ekuhanjisweni kweCas9ribonucleoprotein yeCRISPR/Cas9 genome editing.ITheranostics.2021 Jan1;11(2):614-648.doi: 10.7150 / thno.47007.PMID: 33391496;I-PMCID: PMC7738854.
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