Kumlo ochasene nobhubhane ekuqaleni kuka-2020, kwiintsuku nje ezingama-35, imodeli yempuku ye-ACE2 yasekwa, kwaye umphandi uGuangming Wu kunye noogxa bakhe beZiko leCell Fate kunye noPhando lweLineage (CCLA) kwiBio-Island Laboratories ngempumelelo impumelelo enkulu kusetyenziswa itekhnoloji ye-stem cell ukwenza "umlo ochasene neNew Coronary Pneumonia".Ummangaliso wesantya kuhlaselo olungxamisekileyo.
Uvavanyo lwesiquphe
Ngo-Agasti ka-2019, uWu Guangming, umphandi wexesha elide kummandla wophuhliso lwe-embryonic, wabuyela e-Guangzhou esuka eJamani ukuze ajoyine ibhetshi yokuqala "yePhondo laseGuangdong ukwakha iqela lesizwe lebhubhoratri yelizwe" yeLebhu ye-Bio-Island, eyile. Guangzhou Guangdong Laboratory of Regenerative Medicine kunye neMpilo.
Into ebengayilindelanga kukuba bekungazukuthatha xesha lide ngaphambi kokuba ajongane novavanyo olungalindelekanga lwesithsaba esitsha sokuqhambuka kwenyumoniya.
"Icandelo lophando endibandakanyeka kulo ngokwenene alinanto yakwenza nezifo ezosulelayo, kodwa phambi kobhubhane oluzayo, emva kokufunda ukuba iSebe leNzululwazi kunye neTekhnoloji yePhondo laseGuangdong limisele iprojekthi ekhethekileyo yophando olungxamisekileyo kwisithsaba esitsha. ubhubhani wenyumoniya, bendizibuza ukuba ndingenza ntoni ukulwa lo bhubhani xa ilizwe lonke lisebenzisana. ”
Ngokuqonda, uWu Guangming wafumanisa ukuba iimodeli zezilwanyana ezenziwe ngabantu zifuneka ngokukhawuleza kuxilongo kunye nonyango lwe-coronavirus entsha kunye nolawulo lwayo lwexesha elide.Okubizwa ngokuba yimodeli yezilwanyana ezibizwa ngokuba ngumntu kukwenza izilwanyana (iinkawu, iimpuku, njl.njl.) ezineempawu ezithile zezicubu zomntu, amalungu, kunye neeseli ngokuhlelwa kofuzo kunye nezinye iindlela zokwenza iimodeli zezifo, ukufunda iindlela ze-pathogenic zezifo zabantu kwaye ufumane. ezona zisombululo zonyango.
Uhlaselo lwagqitywa ngeentsuku ezingama-35
UWu Guangming uxelele intatheli ukuba zazikho iimodeli zeeseli ze-vitro ngelo xesha kwaye abantu abaninzi babenexhala.Wayeneminyaka emininzi yamava kuphando lwezilwanyana ze-transgenic kwaye wayelungile kwitekhnoloji yembuyekezo ye-tetraploid.Enye yeembono zakhe zophando ngelo xesha yayikukudibanisa itekhnoloji ye-embryonic stem cell kunye netekhnoloji yembuyekezo ye-embryonic tetraploid kunye ukuseka imifuziselo yempuku eyenziwe ngabantu, kwaye yayikhuthaza into yokuba iZiko leCell Fate kunye noPhando lweGenealogy kwiLabhoratri yeBio Island emva koko yayinobuchwephesha beeseli ezikhokelayo. , kwaye kwakubonakala ngathi zonke iimeko zangaphandle zivuthiwe.
Ukucinga yenye into, ukwenza yenye into.
Kunzima kangakanani ukwakha imodeli yempuku esebenzisekayo?Phantsi kweenkqubo eziqhelekileyo, ingathatha ubuncinane iinyanga ezintandathu kwaye idlule kwiinkqubo ezininzi zolingo neempazamo.Kodwa xa ujongene nobhubhani ongxamisekileyo, umntu kufuneka abaleke ngokuchasene nexesha kwaye axhonywe kwimephu.
Eli qela lasekwa ngokwethutyana kuba uninzi lwabantu lwalusele lugodukile ukuya kuNyaka oMtsha waseTshayina.Okokugqibela, abantu abasibhozo abashiyekileyo eGuangzhou bafunyanwa phantsi kweZiko leCell Fate kunye neGenealogy Research umbutho ukwenza iqela lethutyana lohlaselo lemouse.
Ukususela kuyilo lweprotocol yovavanyo ngoJanuwari 31 ukuya ekuzalweni kwesizukulwana sokuqala seempuku ezenziwe ngabantu ngoMatshi 6, iqela lifezekise lo mmangaliso wophando lwezenzululwazi ngeentsuku ezingama-35 kuphela.Itekhnoloji eqhelekileyo ifuna ukuxuba iiseli ze-mouse stem kunye ne-embryos ukufumana iimpuku ze-chimeric, kwaye kuphela xa iiseli ze-stem zihlula kwiiseli ze-germ kwaye zidibana nezinye iigundane ukuze zidlulisele imfuza ehleliweyo kwisizukulwana esilandelayo seempuku zinokuthathwa njengempumelelo.Iimpuku ezenziwe ngabantu ezisuka kwi-CCLA zazalelwa ukufumana iimpuku ekujoliswe kuzo ngaxeshanye, zafumana ixesha elixabisekileyo kunye nokugcina abasebenzi kunye nezixhobo zokulwa ubhubhane.
UWu Guangming emsebenzini Ifoto/enikezelwe ngudliwano-ndlebe
Zonke zisebenza ixesha elongezelelweyo
UWu Guangming uvumile ukuba ekuqaleni, akukho mntu unentliziyo esezantsi, kwaye itekhnoloji yetetraploid ngokwayo yayinzima kakhulu, ngezinga lempumelelo elingaphantsi kwe-2%.
Ngelo xesha, bonke abantu babezinikele ngokupheleleyo kuphando, kungakhathaliseki imini nobusuku, ngaphandle kweentsuku zokusebenza neempelaveki.Yonke imihla ngentsimbi yesi-3:00 okanye eyesi-4:00 kusasa, amalungu eqela axubusha inkqubela yosuku;bancokola kwade kwasa kwaye ngoko nangoko babuyela kolunye usuku lophando.
Njengenkokeli yobuchwephesha yeqela lophando, uWu Guangming kufuneka alungelelanise imiba emibini yomsebenzi - ukuhlelwa kofuzo kunye nenkcubeko ye-embryo - kwaye kufuneka alandele inyathelo ngalinye lenkqubo yovavanyo kunye nokusombulula iingxaki ngexesha elifanelekileyo, elinoxinzelelo ngakumbi kunomntu. khawufane ucinge.
Ngelo xesha, ngenxa yeholide yoMnyhadala weNtwasahlobo kunye nobhubhani, zonke ii-reagents ezifunekayo zaziphelile, kwaye kwafuneka sifumane abantu kuyo yonke indawo ukuze sibaboleke.Umsebenzi wemihla ngemihla wawukuvavanya, ukuzama, ukuthumela iisampuli kunye nokukhangela ii-reagents.
Ukuze ukhawuleze ixesha, iqela lophando laphula imeko yesiqhelo yenkqubo yovavanyo, ngelixa ukulungiswa kwangaphambili kwenyathelo ngalinye lovavanyo olulandelayo.Kodwa oku kuthetha ukuba ukuba kukho into engahambi kakuhle kumanyathelo angaphambili, amanyathelo alandelayo alungiselelwe ngeze.
Nangona kunjalo, imifuniselo yebhayoloji ngokwayo yinkqubo efuna ulingo oluqhubekayo kunye neempazamo.
UWu Guangming usakhumbula ukuba kanye, i-in vitro vector yayisetyenziselwa ukufaka kulandelelwano lwe-DNA yeselula, kodwa ayizange isebenze, ngoko ke kwafuneka ahlengahlengise ugxininiso lwe-reagent kunye nezinye iiparamitha ngokuphindaphindiweyo kwaye ayenze kaninzi de isebenzile.
Umsebenzi wawunzima kangangokuba wonke umntu wayexakeke kakhulu, amanye amalungu ayenamadyunguza emlonyeni, abanye babediniwe kangangokuba babenokugqushalaza phantsi ukuze bathethe kuba babengakwazi nokuphakama.
Ukuphumelela, uWu Guangming, nangona kunjalo, wade wathi unethamsanqa lokudibana neqela labadlali beqela elibalaseleyo, kwaye kwaba kuhle ukugqiba ukwakhiwa kwemodeli yempuku ngexesha elifutshane.
Usafuna ukuphucula ngakumbi
Ngomhla wesi-6 kuMatshi, iimpuku zesizukulwana sokuqala ezili-17 zazalwa ngempumelelo.Nangona kunjalo, oku kunokuchazwa kuphela njengenyathelo lokuqala ekugqityweni komsebenzi, oye walandelwa ngokukhawuleza yinkqubo yokuqinisekisa ngokungqongqo kunye nokuthunyelwa kweempuku ezenziwe ngabantu kwilebhu ye-P3 ukuze kuhlolwe ngempumelelo intsholongwane.
Nangona kunjalo, uWu Guangming wacinga ngophuculo olongezelelweyo kwimodeli ye mouse.
Uxelele iintatheli ukuba iipesenti ezingama-80 zezigulana ezine-COVID-19 zine-asymptomatic okanye zigula kancinci, nto leyo ethetha ukuba banokuthembela ekuzikhuseleni kwabo ukuze baphile, ngelixa ezinye iipesenti ezingama-20 zabaguli baba nesifo esiqatha, ikakhulu kubantu abadala okanye abo banezifo eziphantsi. .Ke ngoko, ukuze kusetyenziswe ngokuchanekileyo nangempumelelo imifuziselo yeempuku kwi-pathology, ichiza, kunye nophando lwesitofu sokugonya, iqela lijolise kwiimpuku ezenziwe ngabantu kunye nokuguga kwangaphambili, isifo seswekile, uxinzelelo lwegazi, kunye neminye imifuziselo yesifo esisiseko ukuseka imodeli yegundwane yesifo esibi.
Xa ejonga emva kumsebenzi onzima, uWu Guangming uthe uyazingca ngeqela elinjalo, apho wonke umntu wayekuqonda ukubaluleka kwento abayenzayo, enenqanaba eliphezulu lokuqonda, kwaye wasebenza nzima ukuphumeza iziphumo ezinjalo.
Amakhonkco eendaba ayeleleneyo:"I-Guangdong War Epidemic to Honor Heroes" Iqela likaWu Guangming: Iintsuku ezingama-35 zokuseka imodeli yempuku ye-ACE2 (baidu.com)
Ixesha lokuposa: Aug-02-2023