ITekhnoloji yesiZukulwana esilandelayo iKhabazisa ukuDalwa koMfuziselo weMouse
Itekhnoloji yeTurboMice™
Iphuhliswe nguMingCeler ngothotho lokulungelaniswa kwe-tetraploid.
NgokudibanisaItekhnoloji yokuHlela iGene echanekileyokunye nempuku elungiselelweItekhnoloji yokuLungiselela iiseli ze-Embryonic Stem, singahlela ngoku phantse nayiphi na indawo ekujoliswe kuyo yemfuza.
Itekhnoloji yeTurboMice™
Itekhnoloji yethu yeTetraploid yokuZaliswa kweTetraploid iye yasivumela ukuba sandise kakhulu izinga lokuzalwa kweempuku ukusuka kwi-1% -5% ukuya kwi-30% -60% kunye neeseli ze-stem ezihlelwe ngofuzo, eziphantse zifanise ukusebenza kakuhle kokudluliselwa kwe-embryo eqhelekileyo.
I-MingCeler yinkampani yokuqala ehlabathini ukufezekisa ngempumelelo i-Tetraploid Complementation yetekhnoloji ukusuka kwilabhoratri ukuya kwisicelo semizi-mveliso.
ITekhnoloji yesiNtu
Ubuchwephesha bemodeli yezilwanyana zemveli, ezifana ne-pronuclear microinjection kunye nokujoliswa kwe-ES, kubandakanya ukuzala ubuncinane kwi-2 kwizizukulwana ze-3 ukufumana iigundane ze-homozygous genotype, ehlala ithatha iinyanga ezi-6-8.
Le nkqubo inde iphazamisa kakhulu inkqubela kunye nokusebenza kakuhle kophuhliso lwamachiza amatsha kunye nophando lwezifo.
Iqela elikhawulezayo
1. ukuya kuthi ga kwi-20 ye-homozygous gene-edited impuku ngaphakathiIinyanga ezi-2-4.
2. ≈ I-50 yeempuku ze-homozygous phakathi kwezi zilandelayoIinyanga ezi-2.
3. 400+ homozygous iimpuku ngaphakathi8-12 iinyanga.
Ingxelo yeCase ngo-2020
Itekhnoloji yeTurboMice™ yaxhatshazwa ukuvelisa ngokukhawuleza500 homozygousiimpuku ze-ACE2 ezenziwe ngabantu ngaphakathi njeIinyanga ezisi-8kwiyeza le-COVID-19 kunye nophononongo lwesitofu sokugonya.
UKhetho loxinzelelo oluguquguqukayo
Ubuchwephesha bemveli bunemida ebalulekileyo ekukhetheni uhlobo lwempuku, ngelixa itekhnoloji yeTurboMice™ ibonelela ngokuguquguquka okuthe kratya ngeendlela ezahlukeneyo zokuzala kunye neziphuma ngaphandle ongakhetha kuzo (kubandakanya iBalb / c, ICR, C57BL/6, njl.).
● Iimpuku ezivelisa i-F0 ezakhiwe kusetyenziswa ubuchwepheshe be-TurboMice™ ziimpuku ekujoliswe kuzo ezinemvelaphi yeseli enye.
● Ngoko ke, imathiriyeli yemfuzo yeempuku ze-F0 iyafana, inciphisa iimpazamo zokulinga ezibangelwa kukwahluka kwemathiriyeli yemfuzo.
● Ngoko ke, idatha yovavanyo yovavanyo lokuphumelela kwechiza kunye neminye imifuniselo iyangqinelana kwaye ithembekile.
Ugcina Imfezeko yeMfuzo eLungileyo
KwiSitu Precision Genediting
● Ukuhlelwa kwemfuza okuchanekileyo kwindawo echanekileyo kunye namanqanaba achanekileyo embonakalo yemfuza kunye neenkcukacha zezicubu ezichanekileyo.
● Iimpuku ze-ACE2 ezenziwe ngobuntu eziphuhliswe ngobuchule bemveli ziveliswa kukwaziswa kwangaphandle komxhasi we-K18-ACE2, ongakwaziyo ukufezekisa ukuhlelwa okuchanekileyo ngenxa yofakelo olungacwangciswanga, olukhokelela ekunqongophaleni kokuchazwa kwezicubu ze-ACE2 kwimodeli eyakhelweyo ye-ACE2 yempuku. .
● Iimpuku ze-ACE2 zobuntu ze-MingCeler zibonisa imbonakalo ethile kumalungu ahlukeneyo (imizobo C kunye no-D apha ngasentla), kwaye ilinganisa ngokufanelekileyo iimpawu zonyango ezilandela usulelo lwe-SARS-CoV-2.
Iqonga leEnhancerPlus elahlukileyo
●Iqonga le-MingCeler lobunikazi be-EnhancerPlus linokunceda abathengi bethu baphucule amanqanaba embonakalo yemfuza eyenziwe ngabantu.
● Isiphumo seminyaka yokuqokelela idatha kwi-epigenetics kunye nophando lokuzimisela kweseli, iqonga le-MingCeler's EnhancerPlus linokuqikelela ngokuchanekileyo izikhundla ze-genomic ze-Enhancers, okwenza uyilo lwezicwangciso zokuhlela i-gene zisekelwe kubungqina.
● Ngenxa yoko, imfuza ekujoliswe kuyo inokufana ngcono neepateni zokuthetha kunye namanqanaba emfuza engapheliyo.
Ingxelo yetyala: Ukwenziwa komntu kwi-X gene.
● Ukusetyenziswa kweqonga le-EnhancerPlus, isicwangciso-qhinga saphuculwa kwaye inqanaba lokubonisa labona ukunyuka kwee-odolo ezintathu zobukhulu (jonga umzobo ongentla), ukufikelela kwiimfuno zophuhliso lwamachiza njengoko kunqunywe ngumthengi kwinqanaba leprotheni.
● Emva kokugqiba ukuveliswa kobunzima bokuqala kwe-ACE2 yeempuku ngo-2020, i-MingCeler iye yaphucula imodeli yempuku eyenziwe ngobuntu ye-ACE2 ngokuphindaphinda kane ngokusebenzisa i-EnhancerPlus yokuphucula, kunye nenqanaba lokubonisa le-ACE2 eyenzelwe umntu ekugqibeleni ifikelele kufutshane nembonakalo ye-ACE2 yempuku.
Amanqanaba enkcazo ye-ACE2 eyenziwe ngumntu kumalungu ahlukeneyo emodeli yempuku
Multi-Locus Gene Editing
● Iteknoloji ye-TurboMice™ lolona khetho lungcono kwimarike yokuvelisa iimodeli zemouse ezidityanisiweyo zegene-locus ezininzi, inika iingenelo zokuchaneka okuphezulu ekufakeni indawo, ukufakwa kweqhekeza elide, kunye neloci eguqulwe ngofuzo kaninzi, kungekho miba yocalucalulo.
● Itekhnoloji yeTurboMice™ ivumela ukuhlelwa kwangaxeshanye ukuya kuthi ga kwi-3 yemfuza ukuvelisa iimodeli zemouse ehlelwe yijini ye-homozygous ye-multi-locus ngokuthe ngqo kwiiseli ze-embryonic stem ezihlelwe ngofuzo, ngaphandle kwesidingo senkqubo yokuzala/yokuhlola ethatha ixesha, ibonelela ngeendlela ezahlukeneyo. yexabiso eliphezulu leemodeli ezintsonkothileyo ezifunekayo kuphando lweziyobisi oluyilayo.
● I-MingCeler iphuhlise ngempumelelo imifuziselo yemouse eyohlukeneyo ye-loci gene-edited kwithuba elifutshane, esekwe kwi-ACE2 yeempuku.
Iqhekeza elide lokuHlela iGene
Itekhnoloji ye-TurboMice™ yenza kube lula ukuhlelwa kwemfuza okuchanekileyo kwamaqhekeza amade angaphezu kwe-20kb, iququzelela ukuveliswa okukhawulezayo kweemodeli ezintsonkothileyo ezinje ngobuntu, i-conditional knock-out (CKO), kunye neqhekeza elikhulu lokunkqonkqoza (KI).
Iimpapasho
[1] Wang G, Yang ML, Duan ZL, Liu FL, Jin L, Long CB, Zhang M, Tang XP, Xu L, Li YC, Kamau PM, Yang L, Liu HQ, Xu JW, Chen JK, Zheng YT , Peng XZ, Lai R. Dalbavancin ibophelela i-ACE2 ukuvala ukusebenzisana kwayo ne-SARS-CoV-2 spike protein kwaye iyasebenza ekuthinteleni usulelo lwe-SARS-CoV-2 kwiimodeli zezilwanyana.IiRes zeSeli.2021 Jan;31(1):17-24.doi: 10.1038/s41422-020-00450-0.( UKUBA: 20.507 )
[2] Liu FL, Wu K, Sun J, Duan Z, Quan X, Kuang J, Chu S, Pang W, Gao H, Xu L, Li YC, Zhang HL, Wang XH, Luo RH, Feng XL, Schöler HR , Chen X, Pei D, Wu G, Zheng YT, Chen J. Isizukulwana esiKhawulezayo se-ACE2 imodeli yempuku eyenziwe ngumntu ye-COVID-19 ngokuhambelana ne-tetraploid.Natl Sci Rev. 2020 Nov 24;8(2):nwaa285.doi: 10.1093/nsr/nwaa285.( UKUBA: 16.693 )
UkuHamba kweNkonzo
Ekuphela kwento ekufuneka uyenzile kukusinika imfuneko yokuguqulwa kofuzo kwimodeli yakho yemouse.Ngokusekelwe kwiinkcukacha zakho, siya kudibanisa isicwangciso sokuqala esilungiselelweyo, kwaye emva kweengxoxo ezongezelelweyo kunye nesivumelwano esifanayo, siya kusayina isivumelwano senkonzo yobugcisa.Emva kokuqaliswa kweprojekthi siya kubonelela ngohlaziyo olungexesha malunga nenkqubela phambili.